Articles Biology Articles

Enhanced Isolation of SARS-CoV-2 by TMPRSS2-Expressing Cells

Published time: 11 February 2020

Authors: Shutoku Matsuyama, Naganori Nao, Kazuya Shirato, Miyuki Kawase, Shinji Saito, Ikuyo Takayama, Noriyo Nagata, Tsuyoshi Sekizuka, Hiroshi Katoh, Fumihiro Kato, Masafumi Sakata, Maino Tahara, Satoshi Kutsuna, Norio Ohmagari, Makoto Kuroda, Tadaki Suzuki, Tsutomu Kageyama, Makoto Takeda

Keywords: coronavirus, SARS-CoV-2, VeroE6, outbreak, TMPRSS2


Abstract

A novel beta coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which caused a large respiratory outbreak in Wuhan, China in December 2019, is currently spreading across many countries globally. Here, we show that a TMPRSS2- expressing VeroE6 cell line is highly susceptible to SARS-CoV-2 infection, making it useful for isolating and propagating SARS-CoV-2. Our results reveal that in common with SARS- and Middle East respiratory syndrome-CoV, SARS-CoV-2 infection is enhanced by TMPRSS2.


Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells

 

Reference: https://www.pnas.org/content/117/13/7001

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