Biolinker, a biotech startup based in São Paulo, Brazil, are developing a low-cost high-performance COVID-19 diagnostic test using only locally sourced inputs.By Eduardo Geraque | FAPESP Innovative R&D – Researchers at
Biolinker is incubated at the University of São Paulo’s Center for Innovation, Entrepreneurship and Technology (CIETEC), jointly run by the University of São Paulo (USP) and the Nuclear and Energy Research Institute (IPEN).
The project was one of the first to be selected in a call for applications issued by FAPESP’s Innovative Research in Small Business Program (PIPE) in partnership with FINEP, the Brazilian government’s innovation agency, to fast-track funding for products, services or processes developed by tech startups and small businesses in the state of São Paulo to combat COVID-19.
“Within a few months, we plan to have completed the development of a standardized kit for the detection of IgG circulating antibodies in blood serum produced in a later phase of the disease. The method used is ELISA [enzyme-linked immunosorbent assay],” Mona das Neves Oliveira, principal investigator for the project and founder of the firm, told FAPESP Innovative R&D.
The project will use a cell-free protein synthesis technology developed by the firm over the last two years to accelerate and optimize protein production processes on the basis of an in vitro transcription and translation system. Researchers have used the system to develop a protein that is being tested.
“The tests are going very well,” Oliveira said. “We’ve produced the protein and are now purifying it to avoid false positives and false negatives.”
The group also produces proteins by traditional methods. “We’ll compare the results to see which production system obtains the best antigenicity,” Oliveira said.
The detection of IgG in patient blood samples will be performed using antigens from the nucleocapsid protein, the antigenic fraction of the spike protein used by SARS-CoV-2 to invade human cells by binding to the ACE-2 receptor in the cell membrane.
Through a project supported by FAPESP, the researchers were able to develop and validate an aptamer—a peptide that binds to a target molecule—with strong affinity and specificity for the constant fraction of IgG antibodies.
“We plan to use this aptamer conjugated with biotin [which acts as a coenzyme in purine and carbohydrate metabolism] for detection,” Oliveira said.
The test kit will be low-cost because the aptamer and antigens are cheap to produce by the cell-free method. In addition, the firm already has its own plasmids (DNA molecules that can self-replicate) for protein expression and well-established production protocols.
“It will be a very affordable, quick test that can be used anywhere for epidemiological screening purposes,” Oliveira said. “Negotiations are in progress with companies interested in mass-producing the test kit once it’s been calibrated and approved for general use.”
Ester Sabino, a professor and researcher at the University of São Paulo’s Institute of Tropical Medicine (IMT-USP), is partnering with the project. Sabino led the sequencing of SARS-CoV-2 in Brazil.